Abstract: Seed germination stage is a critical period of the growth and development of hemp, the drought has an important effect on the growth and yield of hemp at this stage. In this experiment, 10 hemp varieties were chosen as the test materials to evaluate the drought resistance at seed germination stage by the method of PEG-6000 simulation. The results showed that 5 hemp varieties of good germination and drought resistance under high osmotic potential were selected. In the evaluation of drought stress on hemp at seed germination stage under different concentrations of PEG, low concentrations of PEG could promote the seed germination, and with the increase of PEG concentration, the stress on seed germination was greater. When the PEG concentration was 20%, the difference of germination index among different varieties was significant, so this concentration could be the appropriate concentration for screening and identification the drought-resistant hemp varieties (lines). Using the membership function value method, 3 hemp varieties in Yunnan showed a strong drought resistance, and‘Yunwan 6’was the best one.