Abstract: To select efficient and safe pesticides and control dragonfly larvae in fishing ponds, acute toxicity tests of three pesticides (trichlorfon, bifenthrin and clothianidin) to last-stadium larvae of Orthetrum were performed respectively. The results showed that LC₅₀ (96 h) values of trichlorfon, bifenthrin and clothianidin to last-stadium larvae of Orthetrum were 0.2506, 0.0012 and 0.0517 mg a.i./L, respectively. Comparing LC₅₀ (96 h) values of the three pesticides to the last-stadium larvae of Orthetrum, freshwater fishes and the tadpole of Rana grylio, and the aquatic ecological characteristics of the three pesticides, clothianidin was recommended as the suitable pesticide for controlling dragonfly larvae in fishing ponds.