Effect of Persistent Drought Stress on Chlorophyll Fluorescence Parameters in Leaves of Oil Palm

doi:10.11924/j.issn.1000-6850.casb15070155

Abstract

Abstract: In order to assess the effect of persistent drought stress on chlorophyll fluorescence parameters in leaves of oil palm, 2-year-old seedlings of oil palm were used as study materials, pot culture was adopted to simulate natural persistent drought. The chlorophyll fluorescence parameters and chlorophyll content were measured. And then the drought resistance was analyzed integrally with the method of subordination function. The results showed that under drought stress, the chlorophyll content, variable fluorescence (Fv), maximum fluorescence (Fm), variable fluorescence and maximum fluorescence ratio (Fv/Fm) of oil palm leaf decreased, but the initial fluorescence (F0) and non-photochemical quenching (NPQ) coefficient increased. This suggested that the photosystemⅡ (PS Ⅱ) was hurt by drought stress, which reduced the original light energy conversion efficiency (Fv/Fm) of PS Ⅱ, restrained photosynthetic electron transport and photosynthetic original reaction process, and increased the heat dissipation of protective effect. The changes of chlorophyll fluorescence parameters and chlorophyll content of the four varieties were different. The adaptability to drought from high to low was in an order as‘Reyou 6’,‘Reyou 8’,‘Reyou 2’and‘Reyou 4’. Therefore, the chlorophyll fluorescence parameters and chlorophyll content could be used as diagnostic index of drought resistance strength for oil palm drought-resistant breeding and related studies.

CLC Number:

S718.43

References

[1]许大全.光合作用效率.上海:上海科学技术出版社,2002:29–109
[2]ASADAK,TAKAHASHI M.Production and scavenging of active oxygen in photosynthesis[M]//KLYE D J,OSMOND C B,ARNTZEN C J.Photoinhibition.Amsterdam:Elsevier,1987:227_–287
[3]朱新广,王强,张其德,等.冬小麦光合功能对盐胁迫的响应[J].植物营养与肥料学报,2002,8(2)_:177_–180.
[4]史正军,樊小林.干旱胁迫对不同基因型水稻光合特性的影响[J].干旱地区农业研究,2003,21(3)_:123_–126 .
[5]孙海锋,战勇,林海容,等.花期干旱对不同基因型大豆叶绿素荧光特性的影响[J].大豆科学,2008,27(1)_:56_–60.
[6]杨晓青,张岁岐,梁宗锁,等.水分胁迫对不同抗旱类型冬小麦幼苗叶绿素荧光参数的影响[J].西北植物学报,2004,24(5)_:812_–816.
[7]刘建福,汤青林,倪书邦,等.水分胁迫对澳洲坚果叶绿素a荧光参数的影响[J].华侨大学学报(自然科学版),2003,24(3)_:305_–309.
[8]陈　丹,张　放.水分胁迫条件下CO2加富对枇杷叶绿素荧光及抗氧化酶的影响[J].浙江农业学报,2004,16(2)_:63_–67.
[9]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学报,1999,16(4)_:444_–448.
[10]许大全,沈允钢.光合作用的限制因素. 北京. 植物生理与分子生物学科学出版社.1997.
[11]韩苗苗,张仁和,朱永波,等.不同玉米品种对干旱胁迫的响应[J].种子,2008,27(10)_:49_–55.
[12]王艺陶,周宇飞,李丰先,等.干旱胁迫对高粱叶绿素荧光参数的影响[J].沈阳农业大学学报,2013,44(4)_:398_–403
[13]陈歆,刘贝贝,韩丙军,等.土壤水分胁迫对槟榔幼苗叶绿素荧光动力学参数的影响[J].热带作物学报,2012,33(2)_:230_–237
[14]吴甘霖,段仁燕,王志高,等.干旱和复水对草莓叶片叶绿素荧光特性的影响[J].生态学报,2010,30(14):3941_–3946
[15] Neil R Baker.Chlorophyll fluorescence a probe of photosynthesis in vivo[J]. Annual Review of Plant Biology,2008, 59_: 89_–113.
[16]胡学华,蒲光兰,肖千文,等.水分胁迫下李树叶绿素荧光动力学特性研究[J].中国生态农业学报,2007,15(1)_:75_–77.
[17]李志博,魏亦农,张荣华,等.棉花不同叶位叶绿素荧光特性初探[J].棉花学报,2005,17(3)_:189_–190.
[18] LU C M ,ZHANG Q D,KUANG T Y. The effects ofwater stress on distribution of excitation energy and efficiency of primary conversion of light energy of photosystemⅡinwheat chloroplasts.Acta Biophysica Sinica11.1995,(1)_:82_–86.
[19]罗　俊,张木清,吕建林,等.水分胁迫对不同甘蔗品种叶绿素a荧光动力学的影响[J].福建农林大学学报(自然科学版),2000, 29(1)_:18_–22.
[20] Souzaa R P Machadoa E C Silva J A B.Photosynthetic gas exchange chlorophyll fluorescence and some associated metabolic changes in cowpea during water stress and recovery. Environmtntal and Experimental Botany.2004, 51(1)_:45_–56.
[21]冯建灿,胡秀丽,毛训甲.叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究,2002,20(4)_:14_–18,30
[22]史胜青,袁玉欣,杨敏生,等.水分胁迫对4种苗木叶绿素荧光的光化学淬灭和非光化学淬灭的影响[J].林业科学,2004,40(1)_:168_–173.
[23]刘瑞显,王友华,陈兵林,等.花铃期干旱胁迫下氮素水平对棉花光合作用与叶绿素荧光特性的影响[J].作物学报,2008,34(4)_:675_–683.
[24]贺仲雄．模糊数学及其应用[M]．天津:天津科学技术出版社,1985.67-70．
[25]蒋明义,郭绍川.渗透胁迫及光照下水稻幼苗叶片光合色素降解过程中O₂的参与[J]. 植物学报,1996,38_:797–802.
[26]李伟,曹坤芳.干旱胁迫对不同光环境下的三叶漆幼苗光合特性和叶绿素荧光参数的影响[J].西北植物学报,2006,26_:266–275.
[27]韩瑞宏,卢欣石,高桂娟,等.紫花苜蓿对干旱胁迫的光合生理响应[J].生态学报,2007,27_:5229–5237.
[28] Maxwell K, Johnson G N.Chlorophyll fluorescence a practical guideS[JS].Journal of experimental botany, 2000 , 51S(345):659S_–668S
[29]关军峰.果品品质研究[M]. 石家庄:河北科学技术出版社,2001,105_–116.
[30]张秋英,李发东,高克昌,等.水分胁迫对冬小麦光合特性及产量的影响[J].西北植物学报,2005,25(6)_:1184_–1190.
[31] Papageorgiou GC, Govindjee. Chlorophyll a fluorescence: a signature of photosynthesis (advances in photosynthesis and respiration). Photosynth Res,2005, 83,101_–105.
[32] Baker Neil R.Chlorophyll fluorescence: a probe of photosyn- thesis in vivo . Annual Review of Plant Biology ,2008,59, 89_–113.
[33]陈建明,俞晓平,程家安.叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].浙江农业学报,2006,18(1)_: 51_–55 .
[34] SCHREIBER V,BILGER W.Ripid assessment of stress on plant leaves by chlorophyll fluorescence measurements[C] //TENHU-MENJD.Plant responsetostress-functional analysis in mediterranean ecosystems.Belin: Springe-Verlag,1986, 27_–53.
[35]张建国,李吉跃,沈国舫.树木耐旱特性及其机理研究[M].中国林业出版社,2000,45_–76.
[36]李晓征,彭峰,徐迎春,等.不同遮荫下多脉青冈和金叶含笑幼苗叶片的气体交换日变化[J]. 浙江林学院学报,2005,22(4)_:380_–384.
[37]钱永强,周晓星,韩蕾, 等.Cd2 胁迫对银芽柳PSⅡ叶绿素荧光光响应曲线的影响[J].生态学报,2011,31(20)_:6134_–6142 .
[38]徐建伟,席万鹏,方憬军,等.水分胁迫对葡萄叶绿素荧光参数的影响[J].西北农业学报,2007,16(5)_:175_–179
[39]杨晓青,张岁岐,梁宗锁,等.水分胁迫对不同抗旱类型冬小麦幼苗叶绿素荧光参数的影响[J].西北植物学报,2004,24(5)_:812_–816
[40]裴斌,张光灿,张淑勇, 等.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报,2013,33(5)_:1386_–1396.