Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (12): 33-37.doi: 10.11924/j.issn.1000-6850.casb15070184

Special Issue: 生物技术 小麦

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Segregation of Near-Isogenic Line of Wheat with Drought Resistance Based on Traits and Molecular Marker

Dong Jianli1, Bai Haibo1, Zhu Yongxing1, Lv Xuelian1, Wang Na2, Li Shuhua1   

  1. (1Ningxia Agricultural Bio-Technological Laboratory, Yinchuan 750002;2Ningxia Medical University, Yinchuan 750002)
  • Received:2015-07-31 Revised:2016-04-13 Accepted:2016-01-25 Online:2016-04-27 Published:2016-04-27

Abstract: The study aims to create the drought resistance near-isogenic lines (NILs) of wheat ‘Ningchun 4’, and provide valuable materials for the NILs application in production and the genetic improvement of drought resistance. Due to the certain heterozygous ratio existing in proper generation (F6 or F7) of the recombinant inbred lines (RILs), NILs separation method was applied based on the traits and the molecular markers. The NILs of ‘Ningchun4’ plants with drought were isolated from RIL-97 of ‘Ningchun4’בNingchun27’, the identification of drought resistance and characteristics indicated that the NILs had strong drought resistance, and other morphological characteristics were basically identical to ‘Ningchun4’. The NILs would be used for the accurate positioning of the drought QTLs. The method based on RILs molecular markers of the proper generation of the recombinant was an effective way to construct NILs rapidly.