Abstract: Jinggangmycin has been approved for controlling the maize sheath blight (caused by Rhizoctonia solani). In order to find a substitute that was safe, efficient and environmental friendly to Jinggangmycin, bacterial suspension of Bacillus subtilis BS-8D which was isolated from sheath of corn plants was used to spray on seedlings and basal part of stem at adult stage. Resistance of BS-8D to rifampicin (BS-8DRif) was tested for its ability to colonize plant roots in soil condition, and screened for its antibiosis in dual culture with other pathogenic fungus. The results showed that BS-8D could control corn sheath blight effectively in greenhouse. BS-8DRif showed that the strain could colonize, propagate and remain high populations for more than ten days in the rhizosphere of corn, and could be transported along with the elongating root and transmitted to stems of maize continuously. By dual culture on PDA medium, BS-8D showed strong ability against Exsero turcicum, Bipolaris maydism and Curvularia lunata. The control test showed BS-8D had potential for further research and development.