Abstract: The study aims to explore the feasibility of palm fiber as a culture substrate for grow bags. The physicochemical properties and the content of mineral elements of palm fiber and coconut tree branny were determined and analyzed by using soil physical and chemical property analysis method, in addition, the palm fiber substrate was adjusted according to its plant growth barrier factors. The total porosity of palm fiber was 74.47%, and the water holding capacity of palm fiber was 294.02%, indicating that both factors were within excellent soilless cultivation substrate scope. Gas-water ratio of palm fiber was the same as that of coconut tree branny, both of them were slightly low, gas-water ratio could be adjusted by adjusting the particle size ratio of the substrates. The pH value of palm fiber was 8.46, which was too high for plant growth, however it could be adjusted by adding equal volume of 0.015 mol/L dilute sulphuric acid. Electronic conductivity value of palm fiber was high, it could be decreased to 1.08 ms/cm by drip washing, palm fiber and coconut tree branny had similar water retention and acetic-alkali buffering capacity, the nutrient retention property of palm fiber was weaker, but its own fertility was better than that of coconut tree branny, the content of mineral elements of palm fiber was similar as that of coconut tree branny. If palm fiber was properly adjusted, it could be used as an organic cultivation substrate.