Abstract: In order to conduct scientific fertilization on flue-cured tobacco seedlings under mulch, the effect of different base fertilizer ratios of organic applied with compound fertilizer on plant growth, tobacco yield and interior quality was studied by field experiment. The results showed that: (1) the development process, agronomic traits and SPAD of the treatment of 70 kg organic base fertilizer applied with 20 kg compound fertilizer were relatively better than that of others; (2) compared with conventional fertilization, organic fertilizer combined with compound fertilizer could increase yield and economic value to different degrees, and the treatment of 70 kg organic base fertilizer had better result; (3) the treatment of 70 and 52.5 kg organic base fertilizer had better leaf weight and leaf area; (4) potassium, nicotine, nitrogen, chlorine content and nitrogenalkali ratio had no significant difference among different treatments, while the total sugar content and reducing sugar content of combined application of organic fertilizer and compound fertilizer were higher than that of conventional fertilization. To sum up, the fertilization adjustment had obvious improving effect, and the treatment of 70 kg organic base fertilizer with combined application of compound fertilizer was the best. The results showed that flue- cured tobacco seedlings transplantation under mulch had different fertilization requirements from that under conventional transplantation, and the fertilization scheme should be adjust.