Abstract: In order to clarify the characteristics of spring frost and its impact on yield of winter wheat in winter wheat area of southern Shanxi, the ten days mean value data of daily lowest temperature in late March and early and middle April from 1990 to 2015 in Linfen were collected from Linfen meteorological bureau and the yield data in winter wheat area from 2012 to 2015 were adopted, to analyze the characteristics of spring frost and its impact on yield. The results showed that the spring frost occurred frequently and severely in winter wheat area of southern Shanxi, more than 80% of the studied years were affected by spring frost. The lowest temperature in late March had little impact on wheat yield in late period, and the mean daily lowest temperature was positively correlated with wheat yield in late period. The spring frost damage was concentrated in the early and middle April. In alert period, the lowest temperature in early April was significantly or extremely and significantly correlated with wheat yield in the late period, and the mean daily lowest temperature was positively or significantly and positively correlated with the yield. The lowest temperature and mean daily lowest temperature of the middle of April was positively or significantly and positively correlated with the yield.