Abstract: In order to study wheat plant traits and yield change with low-temperature freezing under different soil moisture, ‘Zhoumai 22’ was chosen as experimental subject, wheat plant traits and yield in late stage were analyzed under soil moisture of 26%, 18% and 10% with low-temperature freezing by keeping the wheat plant in -6℃ (plant body temperature) low-temperature environment for 4 h in its jointing stage. The results showed that the freezing injury effect on wheat plant height, the minimum stem height, peduncle length, top second peduncle length and biological yield decreased gradually with the increase of soil moisture. When soil moisture was close to 10%, the effect on wheat plant traits significantly decreased. Meanwhile, with the increase of soil moisture, the freezing injury effect on wheat yield decreased gradually. When soil moisture was close to 18%, the wheat yield significantly decreased, and the influence of low-temperature on grain numbers of per spike were great and while it had little effect on spike number and 1000 grain weight. After low temperature treatment, the difference of potential tiller spike number among different soil moisture treatments W (26%), M (18%), D (10%) and CK was obvious (P<0.05), the difference of large spike yield among the treatment of W, CK and M, D was also obvious (P<0.05), while, the difference was not significant among other treatments. The experiment provided technical assistance for preventing and reducing cold disaster and improving wheat production.