Abstract: Anther callus and immature embryos callus from drought- sensitive wheat (Wen6 and Zhou17) were investigated using EMS as mutagens. About 50% relative differentiation rate dose of the mutagens were used as the selective indexes of optimal mutagen concentrations and treatment time. The combination of treatments were as follows: Anther callus treatment with EMS solution 0.20%＋（2h~4h）; Immature embryos callus treatment with EMS solution （0.20%~0.40%）＋（2~4h）. The 224 differentiation plants were planted in the rooting medium added 80g/L PEG, which were differentiated from EMS mutate materials, and 13 drought resistance plants were got. EMS mutation ratio was 5.8%. The best combination of treatment were callus treatment with EMS solution 0.20%＋4h.