Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (17): 20-24.doi: 10.11924/j.issn.1000-6850.casb16020113

Special Issue: 生物技术

Previous Articles     Next Articles

Construction of Isoplasm Allonuclear Near-isogenic Restore Line‘L-Rf6’in HL-CMS Rice

  

  • Received:2016-02-29 Revised:2016-04-08 Accepted:2016-04-25 Online:2016-06-20 Published:2016-06-20

Abstract: To study the interaction between two restorer genes and two CMS genes in Honglian (HL) rice, HL-CMS line‘YTA’was crossed with the restore line‘9311’. Then, F1 was backcrossed repeatedly as the female parent with the corresponding maintainer line‘YTB’as recurrent male parent, and isoplasm allonuclear near-isogenic restore line‘L-Rf6’with‘YTA’in HL-CMS rice was constructed. Based on the sequence of Rf5 and Rf6, we designed the specific primers and tested the restorer gene in‘L-Rf6’. The result indicated that the‘L-Rf6’was constructed successfully. By analyzing the expression level of HL-CMS gene orfh79 in‘L-Rf6’, we found that the expression level of orfh79 in‘L-Rf6’decreased distinctly compared with that in‘YTA’. The construction of the near- isogenic restore line could contribute to research the interaction between sterility genes and restorer genes in HL-rice.