Abstract: In order to study the differentiation abilities of regeneration plants of different organs under different hormone concentrations, vitro leaves, petiole and root segment of Taraxacum kok-saghyz Rodin were used as explants and MS as basic culture medium. The results showed that: the best hormone concentrations for bud induction of different organs of Taraxacum kok-saghyz Rodin were different, (1) NAA 0.2 mg/L 6-BA 0.5 mg/L was most suitable for regeneration by bud induction of leaf and petiole, and the differentiation rate and the number of the average bud per explant were 36.44%, 38.83, 95.83%, 10.56, respectively; (2) NAA 1.0 mg/L 6- BA 0.1 mg/L was most suitable for regeneration by bud induction of root, the differentiation rate and the number of the average bud per explant were 95.25% and 21.49, respectively. The results lay a foundation for the establishment of a high frequency regeneration system and further variety improvement by using gene engineering technology.