Community Structure and Diversity of Airborne Microbes: Different Functional Regions in Spring of Nanjing

doi:10.11924/j.issn.1000-6850.casb16040011

Abstract

Abstract: The study aimed at finding the distribution regularities of atmospheric microbes in different functional regions in Nanjing, thus to provide theoretical evidences for controlling some air-borne pathogenic bacteria. We collected air samples using three methods from three domains (NAU, NBG, XWG) and conducted further analysis. Diversity and concentration of bacterial 16S rDNA and fungal 18S rDNA in three functional areas of Nanjing were analyzed by PCR amplification, gene clone, RFLP (restricted fragment length polymorphisms) and sequence analysis. The results showed that air samples derived from the three domains collected from the spring of Nanjing were dominated by bacteria such as Bacillus, Micrococcus and Pseudomona and Alternaria, Penicillium, Aspergillus and Sistotrema were common fungi as well. Meanwhile, the conditioned pathogens such as serratia marcescens were detected. In general, the abundance and diversity distribution regularities of bacteria as well as fungi in different domains in spring of Nanjing were confirmed, and the study provided a feasible way for air quality monitoring and atmospheric microorganism research in Jiangsu Province and the whole country.

Qu Haoli,Zhao Juan,Xiao Yongliang and Cao Hui. Community Structure and Diversity of Airborne Microbes: Different Functional Regions in Spring of Nanjing[J]. Chinese Agricultural Science Bulletin, 2016, 32(32): 174-181.

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