Abstract: Studies on hormone combinations of 3 kinds of culture including primary culture、subculture and rooting culture in the micropropagation method of Oxalis triangularis purpurea also on the transplanting conditions of rooted shoots.The experiments showed that the adventitious buds were induced to proliferation on MS medium supplemented with 6-BA 0.5 mg/L、NAA0.5 mg/L and 2,4-D 0.1 mg/L,the induction rate was 83%. On an average 2.6 shoots were formed per original explant in the initial culture.The number of shoots in subculture was more than 3 on MS medium supplemented with 6-BA 0.5 mg/L and NAA0.1 mg/L.The rooting rate of the shoots reached 92% on 1/2MS medium supplemented with 0.1 mg/L NAA;After transferred to a mixture with vermiculite and sands (1:1),about 90% of the rooted shoots were survival.