Abstract: An efficiency system of propagation in vitro of Phlox paniculata.L.was developed. The optimum sterilization of young shoots was 9 minutes with 0.1% HgCl2. The best effect of explants was cultured on MS +BA1.0+NAA0.1 +30g sugar and the best effect of multiplication was cultured on MS +BA0.5+ IBA 0.3 +30g sugar. The survival rate of transplant was the hightest by rooting in nurery and the propagation periods were the shortest.