Abstract: In order to evaluate tourism climate resources value of Guide County in the Yellow River Valley of Qinghai and to ensure an healthy and sustainable development of tourism industry, based on the daily meteorological data observed by Guide County meteorological station from 1961 to 2015, the change characteristics of comfort index of human body and their influence factors were analyzed. The results indicated that: the main comfort degrees in the Yellow River Valley were frigid, cold, cool, pleasantly cool and comfortable, the overall character was cold and cool; there were significant differences of annual average days of each degree; January and December were the most uncomfortable months, while July was the most comfortable month, followed by August and June; ICHB increased significantly in the Yellow River Valley from 1961 to 2015, ICHB of four seasons increased significantly; seasonal differences of ICHB were significant, the relationship of each season was summer＞autumn＞spring＞winter.