Abstract: To provide a theoretical basis for flowering regulation of hybrid Cymbidium, the effects of 6-BA, GA3, spraying times and spraying interval on floral bud differentiation and flowering quality of hybrid Cymbidium‘Hongmeiren’were studied with an orthogonal experimental design. The results indicated that 6- BA GA3 facilitated the floral bud differentiation of hybrid Cymbidium, and the optimum treatment was 6-BA 200 mg/L GA3 200 mg/L spraying 2 times every 5 days, the number of flowering stalks were up to 6.42 per pot. 6-BA concentration was the most important factor influencing the number of flowering stalks per pot, followed by spraying interval, spraying times and GA3 concentration. Spraying times was the most important factor influencing the number of flowers per stalk, followed by GA3 concentration, 6-BA concentration and spraying intervals.