Abstract: Through the growth rate of rice callus research, find train temperature to the growth rate of rice callus to be of great impact , Smaller temperature is it can make the Growth Rate of Rice Callus reduce a lot of to reduce, The growth rate of rice callus with continuing while taking the place of cultivation in succession , relate to change 2，4-D density, Generally shown as and changed 2，4-D density to continue taking the place of under lower temperature，Can promote the Callus of rice and grow; Change 2,4-D density to continue taking the place of under higher temperature, he Growth Rate of Rice Callus to be influence strong. Train it under the condition in 23-25 degrees Centigrade, The monoploid that the rice anther train Callus to "zhonghua11 ", than maturity liploid that embryo lead of it,s callus grow speed to be one times faster and about. When is it take the place of to continue initial to inoculate amount of quantity the Growth Rate of Rice Callus have relation too.