Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (30): 43-50.doi: 10.11924/j.issn.1000-6850.casb16090028

Previous Articles     Next Articles

Identification and Analysis of Steviol Glycosides in Stevia Rebaudiana Leaves by HPLC-MS

  

  • Received:2016-09-06 Revised:2016-11-09 Accepted:2016-11-25 Online:2017-10-31 Published:2017-10-31

Abstract: To improve the measurement method of steviol glycosides (SGs) in Stevia leaves, several SGs standards and leaf extracts of‘Xinfeng 3’were analyzed and determined by using high performance liquid chromatography-mass spectrometer (HPLC-MS). And the retention time and order of 12 different SGs were identified, among which three (rubusoside, steviolbioside and rebaudioside E) were first reported. Based on the rebaudiodside A standard curve and HPLC fingerprints map of SGs, the composition of SGs in‘Xinfeng 3’ leaves was determined. Totally nine kinds of SGs including stevioside, rebaudioside A, B, C, D, E and F, rubusoside and steviolbioside were identified. The rebaudioside D and E were presented in trace amount. This study provides not only detailed ultraviolet-map for further detection of SGs, but also a reference method for finding and identifying new SGs.