Abstract: 60 healthy chickens were divided into two groups, immunological inhibition and control. After 3 days injected, the chickens were drew blood to cultivate the full blood by whole blood method and MTT method. Taking supernatant and centrifuging on OD570nm at 24h, 48h, 72h, 96h and 120h. MTT is the same as the last. Detecting the OD570nm value of the supernatant at 72h and the cell suspension of MTT method at 48h, indicating twice at the same condition after mixing and the next day indicating again. As a result: The OD570nm value of supernatant and MTT all reflect the condition of the lymphocyte transformation. The repeatability of the whole blood method is better than the MTT.