Target gene expression and stem borer resistance of insect-resistant super japonica rice transformed with cry1C gene in field

doi:10.11924/j.issn.1000-6850.casb17040038

Abstract

Abstract: To study the target gene expression and stem borer resistance in transgenic insect-resistant super Japonica rice in field, 10 super rice strains with insect-resistant genes Japonica rice transformed with cry1C* from different independent resistant callus were grown in field. The mRNA of target gene in rice leaf, stem sheath and young panicle at booting stage were detected by real-time PCR. The Cry1C protein in rice leaf, stem sheath, young panicle at booting stage and in brown rice after harvest were detected by enzyme-linked immunosorbent assay (ELISA). The white head plants damage of Chilo suppressalis was investigated by visual inspection in field. The results showed that the expression of cry1C* in different organs and different transgenic super Japonica rice strains was obviously different. The quantities of mRNA expression of cry1C* gene followed an order: leaf>stem sheath>young panicle, and the quantities of cry1C* protein followed an order: leaf>stem sheath>young panicle> brown rice. The positive correlation of target gene expression in transgenic super Japonica rice existed in different organs at mRNA and protein levels. The positive correlation existed between Cry1C protein content in organs and Bt protein content in brown rice. The stains, no matter with high or low Cry1C protein contents, had high resistance to Chilo suppressalis in filed in our study. The insect resistant genotype which properly expressed target genes should be paid attention in breeding transgenic insect-resistant Japonica varieties.

李冬雪,田崇兵,刘长华 and 李荣田. Target gene expression and stem borer resistance of insect-resistant super japonica rice transformed with cry1C gene in field[J]. Chinese Agricultural Science Bulletin, 2017, 33(36): 131-138.

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