Identification and Molecular Characteristics of Vibrio harveyi from Fenneropenaeus chinensis

doi:10.11924/j.issn.1000-6850.casb17050026

Abstract

Abstract: In this study, the dominant strain was isolated from diseased Fenneropenaeus chinensis , the biochemical and molecular biological identification were conducted for this strain. The strain was green on TCBS medium (thus designated as TCBS-G). Based on physiological and biochemical analysis, TCBS-G was identified to be Gram-negative, in short rod shape, blunt ends, and with flagellum. Biolog GIII experiment showed that the growth and metabolism of TCBS-G were similar to that ofVibrio harveyi . Homology of TCBSG withVibrio spp. was analyzed based on the sequences of 16S rRNA gene,gyrB andHSP60 . Based on the sequence of 16S rRNA gene, TCBS-G was not identified. Phylogenetic tree based ongyrB andHSP60 genes showed that TCBS-G clustered together withV. harveyi , with a high bootstrap value of 99%. In summary, all above results confirmed that the isolated strain TCBS-G wasV. harveyi . The substrate plate test showed that it could produce diastase, proteinase, lipase, and DNase. In addition, the conserved region in hemolysin gene of V. harveyi could be amplified by PCR with specific primers.

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