Abstract: The paper aims to build callus induction and plantlet regeneration system for Uncaria rhynchophylla (Mip.) Jack. The authors took the fresh stems, shoots and leaves as explants to conduct the induction and differentiation of callus, and the rooting and transplantation of seedlings by optimizing hormone combination, to establish the callus induction and plantlet regeneration system of Uncaria rhynchophylla (Mip.) Jack. The results showed that: the callus of the fresh shoot of Uncaria rhynchophylla (Mip.) Jack as explant was better, its pollution rate was low, and the callus growth was faster after subculture; the optimal medium for callus induction was WPM+2.0 mg/L 2,4-D+0.5 mg/L 6-BA; for re-differentiation WPM+2.0 mg/L KT+0.2 mg/L NAA; and for rooting 1/2WPM+1.0 mg/L IBA; the callus induction, re-differentiation and rooting rate was 83.3%, 82.3% and 92%, respectively. In conclusion, the callus is induced by the explants of fresh shoots and the plants are regenerated successfully, and the results obtained can be used to solve the resource and quality problems of the artificial cultivation of Uncaria rhynchophylla (Mip.) Jack, and to lay a foundation for cell culture and molecular genetic improvement.