Selection and Biological Characteristics of a Lead Resistant Strain

doi:10.11924/j.issn.1000-6850.casb17070135

Abstract

Abstract: For the problem of soil heavy metal contamination in Shanxi, we screened lead resistant strains to provide the ideal one for microbial remediation. We isolated the lead resistant strains from heavy metal contaminated soil by using the selective medium, and identified the strains by physiological and biochemical tests and the 16S rDNA sequence analysis. At last, we screened out a lead resistant strain named GDYX03 that belonged to Enterobacter. The optimum growth condition of the strain was found as inoculation quantity of 10%, pH 6.0, 30℃ and the ventilation volume of 10 mL. Under this condition, the strain GDYX03 possessed the capability of Pb2+ resistance up to 2000 mg/L, and its adsorption rate and quantity of Pb2+ reached 98.88% and 19.78 mg/g in aqueous solution, respectively. The adsorption effect of this strain on lead is significant, and it could be used for microbial remediation of soil heavy metal contamination.

郜春花,,,, and . Selection and Biological Characteristics of a Lead Resistant Strain[J]. Chinese Agricultural Science Bulletin, 2018, 34(14): 20-27.

References

[1] Tsezos M. Metal-Microbes Interactions: beyond Environmental Protection[J]. Advanced Materials Research, 2009, 71-73(1):527-532.
[2] Catriona A. Macdonald,Ian M. Clark,Penny R. Hirsch, et al. Long- term impacts of zinc and copper enriched sewage sludge additions on bacterial, archaeal and fungal communities in arable and grassland soils[ J] . Soil biology & biochemistry,2011,43(5):932-941.
[3] Steven Alan Wakelin,Guixin Chu,Kris Broos, et al. Structural and functional response of soil microbiota to addition of plant substrate are moderated by soil Cu levels[ J] . Biology and Fertility of Soils, 2010 , 46 ( 4 ) : 333 - 342 .
[4] 王皙玮,王秋红,於丽华. 土壤重金属污染及对生物体影响的研究进展[J]. 中国农学通报,2017, 33(19).
[5] 郑师章,吴干红,等.普通生态学-原理、方法和应用[M].上海：复旦大学出版社,1994.
[6] 李坤陶.生物修复技术及其应用[M].生物学教学,2007,32(1):4-6.
[7] 林晓燕,牟仁祥,曹赵云,等. 耐镉细菌菌株的分离及其吸附镉机理研究[J]. 农业环境科学学报, 2015,34(9)：1700-1706.
[8] 徐磊辉,黄巧云,陈雯莉. 环境重金属污染的细菌修复与检测[J]. 应用与环境生物学报,2004,10(2):256-262.
[9] 李晶, 刘玉荣, 贺纪正,等. 土壤微生物对环境胁迫的响应机制[J]. 环境科学学报, 2013, 33(4):959-967.
[10] Massaccesi G, Romero M C, Cazau M C, et al. Cadmium removal capacities of filamentous soil fungi isolated from industrially polluted sediments, in La Plata (Argentina)[J]. World Journal of Microbiology and Biotechnology, 2002, 18(9):817-820.
[11] 金羽, 曲娟娟, 李影,等. 一株耐铅细菌的分离鉴定及其吸附特性研究[J]. 环境科学学报, 2013, 33(8):2248-2255.
[12] Volesky B, Holan Z R. Biosorption of heavy metals [J]. Biotechnology Progress, 1995, 11(3):235-250.
[13] 陈素华,孙铁珩,周启星,等. 微生物与重金属间的相互作用及其应用研究[J].应用生态学报,2002,13(2)：239-242.
[14] 赵斌,何绍江.微生物学实验[M].北京：科学出版社. 2002:75-159.
[15] 朱艳蕾. 细菌生长曲线测定实验方法的研究[J]. 微生物学杂志, 2016, 36(5):108-112.
[16] 周赓,杨辉,潘虎,等.一株耐镉链霉菌的筛选、鉴定与基本特性分析[J].环境科学学报,[2016-12-16]. http://www.cnki.net/kcms/detail/11.1843.X.20161216.1456.001.html.
[17] 王政, 魏莉. 利用 SPSS 软件实现药学实验中正交设计的方差分析[J]. 数理医药学杂志, 2014(1): 99-102.
[18] 姚玲丹、王丽晓、朱亮,等.一株壬基酚降解菌的分离鉴定及其对壬基酚同分异构体降解特性研究[J].环境科学学报,[2017-01-03]. http: // www, cnki. Net/kcms/detail/11. 1843. X. 20170103. 1556. 001. html.
[19] 冯伟华, 常艳璐. 长双歧杆菌液态发酵条件的优化及生长曲线的测定[J]. 中国医药导报, 2012, 9(31):115-117.
[20] 周小红, 李学英, 杨宪时,等. 接种量对单增李斯特菌生长/非生长界面的影响[J]. 中国食品学报, 2016(12):75-85.
[21] 梁锦锋. 解磷细菌(Bacillus megaterium var.phosphaticum)生长条件及解磷机理的研究[D]. 杭州:浙江大学, 2001.
[22] 肖春文. 耐镉细菌的筛选及其耐镉特性的初步研究[D]. 湖南农业大学, 2014.
[23] 刘爱民. 耐镉细菌筛选与吸附镉机理研究及其在镉污染土壤修复中的应用[D]. 南京:南京农业大学, 2005.
[24] Amoroso M J, Castro G R., Durán A, 2001. Chromium accumulation by two Streptomyces spp, isolation from riverine sediments [J]. Journal of Industrial Microbiology and Biotechnology, 26: 210-215.
[25] Geoffrey M G. 2000. Bioremedial potential of microbial mechanisms of metal mobilization and immobilization [J]. Current Opinion in Biotechnology, 11: 271-279.
[26]刘丽,李湘凌,范丽,等.铜陵新桥矿区土壤中耐Cu微生物的筛选研究[J].生态环境学报,2010,19(1):118-122.
[27]刘爱民,黄为一.耐镉菌株的分离及其对Cd2+的吸附富集[J].中国环境科学,2006,26(1):91-95.
[28]江春玉. 重金属铅镉抗性菌株的筛选、生物学特性及其强化植物修复铅镉污染土壤的研究[D]. 南京:南京农业大学, 2005.
[29]李春喜,王志和,王文林2002．生物统计学[M]北京: 科学出版社.
[30]王春耀,张德纯,张名均,等. 两歧双歧杆菌86321 生长特性的研究[J].中国微生态学杂志,2011,23(8): 706-711．