Abstract: This study elaborates a new species in a karst cave near the foot of Longshan Mountain, Xiaoxinzhai Village, Shupi Township, Qiubei County, Wenshan Zhuang and Miao Autonomous Prefecture, Yunnan Province, China. The new species is a member of Sinocyclocheilus, and named as Sinocyclocheilus longshanensis Li et Wu sp. nov. according to the name of collection region. Morphological observation shows that the new species has 7 branched dorsal-fin rays, trailing edges of the last unbranched dorsal-fin ray is serration; normal eyes; sub-upper mouth; humped back; black-brown round spots distributed on exposed body surface; 59~62 lateral line pores. The new species can be distinguished from Sinocyclocheilus maculayus, which has humped back; black-brown round spots distributed on exposed body surface; 81~88 lateral line pores. The new species can be distinguished from Sinocyclocheilus yangzongensis, which has flat back; golden body color; 71~81 lateral line pores. The new species also can be distinguished from other species of Sinocyclocheilus by having the described combination of morphological characteristics.