Abstract: For determining narigin, hesperidin and neohesperidin in Citrus fruits, a high performance liquid chromatographic (HPLC) method was established. The HPLC condition was as follows: Waters Atlantis T3-C18 column (4.6 mm×150 mm, 3 μm) was used at 40℃ with acetonitrile-water containing 0.5% acetic acid (20:80, v/v) as the mobile phase with a flow rate of 1.0 mL/min, diode array detector with the detection at 283 nm. The linear range of naringin, hesperidin and neohesperidin was 1- 500 μg/mL (r=0.9999), 1- 500 μg/mL (r=1.0000), 1-500 μg/mL (r=0.9999), respectively. The average recovery was all over 95%. The results of methodological study demonstrate that the method could meet the determination requirements, and is convenient and accurate with good reproducibility, could be used to study the content of flavonoids in Citrus fruits.