Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2018, Vol. 34 ›› Issue (4): 123-130.doi: 10.11924/j.issn.1000-6850.casb17090060

Previous Articles     Next Articles

EMS Mutagenesis in Vitro and Screening of Resistant Mutant to Kabatiella microsticta of Hemerocallis middendorfii

  

  • Received:2017-09-11 Revised:2018-02-01 Accepted:2017-11-25 Online:2018-02-26 Published:2018-02-26

Abstract: The calli and differentiation buds from ovary of Hemerocallis hybrida were mutagenized in vitro by different concentrations of EMS (ethyl methane sulfonate) and screened by toxin extracts after the toxicity testing of Kabatiella microsticta on Hemerocallis hybrida. The results showed that 0.50%-0.75% EMS treated callus 60 min, 0.75%-1.00% EMS treated differentiation bud 60 min, and obtained semi lethal dose effect respectively. The surviving calli were gradually screened for bacterial toxin concentration (10%, 30 days- 20%, 30 days), and the surviving differentiated buds were screened for 21 days with 40% toxin, one step solution. 103 mutants of leaf blight from bud differentiation were obtained by directional screening, and further identification was needed. Calli failed to obtain resistant mutant plants.