Abstract: In order to explore the current situation and the adaptive environment of Gladiolus gandavensis resources in Cangnan, we carried out the investigation on the habitat of Gladiolus gandavensis by means of sample plots survey. The results showed that Gladiolus gandavensis distribution area in Cangnan mainly concentrated in the southeastern or northwestern slope of Beiguan island, Pucheng and Mazhan. The suitable altitude for Gladiolus gandavensis was between 30 m~100 m, and the most appropriate shading tree species were Casuarina equisetifolia, Zanthoxylum ailanthoides and Pinus thunbergii. In the distribution areas of Gladiolus gandavensis, the malicious weeds (Miscanthus sinensis, Setaria viridis, Artemisia lavandulaefolia, etc.) height were almost between 30 cm~60 cm, which restrict the growth and development of Gladiolus gandavensis. There were few weeds in the habitat of Gladiolus gandavensis in Mazhan, and the fertile and well drained sandy soil were conducive to the growth of Gladiolus gandavensis. Therefore, the optimal average density and growth of Gladiolus gandavensis were in Mazhan. The sharp decreasing of seedlings and soil hardening were the important factors of the weak growth of Gladiolus gandavensis in Beiguan island. This investigation found out that the geographical distribution and degressive causes of Gladiolus gandavensis in Cangnan and provide a scientific basis for the conservation and utilization of Gladiolus gandavensis resources.