Distribution Regularity of Microsatellites in Scleropages formosus Genome

doi:10.11924/j.issn.1000-6850.casb18030101

Abstract

Abstract: This study focused on the distribution of microsatellite in the genome of golden arowana (Scleropages formosus). The quantity and distribution of whole-genome microsatellites of the international endangered species, golden arowana, were analysed based on bioinformatics methods. The results showed that there were 354,239 micrositellite sequences with 1-6 microsatellite repetitions, and the overall length was 6139487 bp. The preponderance of mononucleotide , dinucleotide, trinucleotide, tetranucleotide and pentanucleotide were A, AC, AAT, ATCC and AAAAT respectively. Among the 25 chromosomes, chromosome 6 contained the largest number of microsatellites (28662), and the chromosome 22 had the least (6929). Linear correlation (r=0.999) was observed between the number of microsatellites and the length of DNA sequences they located in. However, chromosome 22 had the highest frequency of microsatellite occurrence（576.35/Mb） and chromosome 3 had the lowest（469.65/Mb）. The result showed that the frequency of microsatellite was not related to the length of the chromosome DNA sequence in which it was located. The research provides help for the further study on genetic relationship and genetic diversity analysis in golden arowana, and the transferbility of the microsatellite markers.

段永楠,,,,,, and 牟希东. Distribution Regularity of Microsatellites in Scleropages formosus Genome[J]. Chinese Agricultural Science Bulletin, 2019, 35(23): 152-158.

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