Abstract: Screening the best media of spore propagation in Osmunda japonica Thund. and understanding fully that spore propagation in Osmunda japonica Thund. required for the environmental factors, we maked use of Osmunda japonica Thund. spores in Hubei Institute for Nationalities and in the city suburb of Hangzhou to process propagative test in forest soil, farm land soil, red sand soil media by overshadowing. The result indicates that spore germination shapes prothallium at keeping wet, cortrolling temperature and covering with 75% shading rate. If continuously culture, all can enter the sexual generation and shape sporophytic young seedling. Under the aspact of the propagating young seedling, the experiment shows forest humic soil: field soil1=1: 1(Volume rate) is controlled most easily among these media. The seedlings in unit area can amount to 3500 plant/m2. Compared with collecting in Hubei Institute for Nationalities, shaded prothallium and sporophyte after collecting Osmunda japonica Thund. spores germinate in Hangzhou both have better heat-tolerance.