Abstract: In order to develop drought-resistance breeding of soybean and carry on regional distribution of soybean planting,aim at the outstanding problems in being short of drought-resistance cultivars、low yields and undefined the types of drought resistance in Heilongjiang Province, from which 40 varieties of spring soybeans were selected to identify the drought-resistance in budding and seedling stages by using PEG-6000 in this paper. Then simulate drought stress on the screened drought-resistant varieties to study the root characteristics including total root length, root surface area、root volume、root and crown ratio. The results were as following： In budding stage, Suinong series、Heinong series 、Hefeng series and xiaoli series showed more drought-resistance contained Suinong 22、Hefeng 48、Suinong 30、Longxiaolidou 2、Heihe 52、Suinong 28、Kendou 26、Heinong 65、Longxiaolidou 1、Kenfeng 17、Kendou 25、Suinong 10、anti-nematode 12、Heinong 61、Heinong 64 and Longdou2.In seedling stage, Hefeng series、Suinong series and Heinong series showed more drought-resistance contained Hefeng 50、Heinong 60、Suinong 28、Hefeng 48、Hefeng 51、Heinong 61、Longxiaolidou 2、Heihe 51、Heihe 50、Hefeng 56、Heinong 65 and Heinong 64. Combine the identification results of drought-resistance with budding and seedling stage, Hefeng 48,Suinong 28、Longxiaolidou 2,Heinong 65,Heinong 61,and Heinong 64 were screened out of which total root length, root surface area、root volume、root and crown ratio expressed the increasing tendency especially from moderate to severe drought with the drought stress intensity enhancing. The strongest performance variety was Hefeng48 and Heinong 61 Heinong 64 and Suinong 28 were stronger.