Farmland Surrounding High Arsenic Pyrite: Distribution of Soil Arsenic and Characteristics of Fungi Community Structure

doi:10.11924/j.issn.1000-6850.casb18030150

Abstract

Abstract: We studied the fungi community in the farmland surrounding the high-arsenic pyrite area, analyzed the community characteristics of fungi in the soils of different regions around the mining area by Miseq highthroughput sequencing technology, in order to clarify the characteristics and adaptation mechanism of the fungi community. The results showed that: (1) arsenic and iron combined contaminated soil could decrease the richness of fungi in soil, and the impact of the combined contaminated soil on fungi diversity was first promoted and then inhibited; (2) arsenic and iron combined pollution inhibited the metabolism of Ascomycota in soil and simulated the metabolism of Basidiomycota, whereas showed a two- phase effects on the Zygomycota, Fusarium, Pseudallescheria and Cryptococcus, namely, at low doses it promoted the metabolism, while at high doses it had a negative effect; (3) arsenic and iron combined pollution caused significant differences in the fungi community structure in soils, and the effects of environmental factors on fungi community structure also had significant differences, the order was total arsenic and total iron. Therefore, during the mining process of high- arsenic pyrite, the fungal community structure in the soil is affected to a certain extent, and it is necessary to strictly manage the stacking of minerals and slag so as to avoid serious damage to the habitat of various organisms in the soil.

References

[1]Coppolecchia D, Puglisi E, Vasileiadis S, et al. Relative sensitivity of different soil biological properties to zinc[J]. Soil Biology Biochemistry, 2011, 43(9): 1798-1807.
[2]Li Z, Ma Z, Van Der Kuijp T J, et al. A review of soil heavy metal pollution from mines in China: Pollution and health risk assessment[J]. Science of the Total Environment, 2014, 468-469: 843-853.
[3]Li J, Ma Y B, Hu H W, et al. Field-based evidence for consistent responses of bacterial communities to copper contamination in two contrasting agricultural soils[J]. Frontiers in Microbiology, 2015, 6: 31.
[4]Borchers J G, Borchers S L, Brainerd R E. Bootstrapping in ecosystems[J]. Bioscience, 1989, (4): 230-237.
[5]Leita L, De N M, Muhlbachova G, et al. Bioavailability and effects of heavy metals on soil microbial biomass survival during laboratory incubation[J]. Biology Fertility of Soils, 1995, 19(2-3): 103-108.
[6]Bonkowski M, Roy J. Soil microbial diversity and soil functioning affect competition among grasses in experimental microcosms[J]. Oecologia, 2005, 143(2): 232-240.
[7]Griffiths B S, Ritz K, Bardgett R D, et al. Ecosystem Response of Pasture Soil Communities to Fumigation-Induced Microbial Diversity Reductions: An Examination of the Biodiversity - Ecosystem Function Relationship[J]. Oikos, 2000, 90(2): 279-294.
[8]Heijden M G a V D, Klironomos J N, Ursic M, et al. Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity[J]. Nature, 1998, 396(6706): 69-72.
[9]Maherali H, Klironomos J N. Influence of phylogeny on fungal community assembly and ecosystem functioning[J]. Science, 2007, 316(5832): 1746-1748.
[10]Bradford M A, Jones T H, Bardgett R D, et al. Impacts of soil faunal community composition on model grassland ecosystems[J]. Science, 2002, 298(5593): 615-618.
[11]Laakso J, Set?l? H. Sensitivity of Primary Production to Changes in the Architecture of Belowground Food Webs[J]. Oikos, 1999, 87(1): 57-64.
[12]张文慧, 许秋瑾, 胡小贞, 等. 山美水库沉积物重金属污染状况及风险评价[J]. 环境科学研究, 2016, 29(7): 1006-1013.
[13]黄瑞卿, 王果, 汤榕雁, 等. 酸性土壤有效砷提取方法研究[J]. 农业环境科学学报, 2005, 24(3): 610-615.
[14]刘永兵, 贾斌, 李翔, 等. 海南省南渡江新坡河塘底泥养分状况及重金属污染评价[J]. 农业工程学报, 2013, 29(3): 213-224.
[15]王亚男. 外源砷在土壤中的老化及其对土壤微生物影响的机理研究[D]. 北京: 中国农业大学, 2016.
[16]周丹, 赵永红, 王春晖, 等. 钨矿区砷污染与土壤微生物及酶活性相关性研究[J]. 有色金属科学与工程, 2013, (6): 57-62.
[17]王薪宇. 砷对土壤微生物数量及土壤生物活性的影响研究[D]. 辽宁阜新: 辽宁工程技术大学, 2014.
[18]宋卫锋, 邓琪, 宾丽英, 等. 外源砷对土壤微生物数量的影响研究[J]. 湖北农业科学, 2011, 50(13): 2636-2638.
[19]Z L, Z M, Tj V D K, et al. A review of soil heavy metal pollution from mines in China: pollution and health risk assessment[J]. Science of the Total Environment, 2014, 468–469: 843-853.
[20]Somerfield P, Gee J, Warwick R. Soft sediment meiofaunal community structure in relation to a long-term heavy metal gradient in the Fal estuary system[J]. Marine Ecology Progress, 1994, 105(1-2): 79-88.
[21]郑景华, 巴楚明, 王志宏, 等. 矿区土壤中砷污染对微生物群落的影响研究[J]. 地球与环境, 2016, 44(5): 506-512.
[22]Deram A, Languereauleman F, Howsam M, et al. Seasonal patterns of cadmium accumulation in Poaceae): Influence of mycorrhizal and endophytic fungal colonisation[J]. Soil Biology Biochemistry, 2008, 40(3): 845-848.
[23]Regvar M, Likar M, Piltaver A, et al. Fungal community structure under goat willows (Salix caprea L.) growing at metal polluted site: the potential of screening in a model phytostabilisation study[J]. Plant Soil, 2010, 330(1): 345-356.
[24]Ban Y, Xu Z, Zhang H, et al. Soil chemistry properties, translocation of heavy metals, and mycorrhizal fungi associated with six plant species growing on lead-zinc mine tailings[J]. Annals of Microbiology, 2015, 65(1): 503-515.
[25]Val C D, Barea J M, Azconaguilar C. Diversity of arbuscular mycorrhizal fungus populations in heavy-metal-contaminated soils[J]. Applied and Environmental Microbiology, 1999, 65(2): 718-723.
[26]Zarei M, Konig S, Hempel S, et al. Community structure of arbuscular mycorrhizal fungi associated to Veronica rechingeri at the Anguran zinc and lead mining region[J]. Environmental Pollution, 2008, 156(3): 1277-1283.
[27]Yang R, Zan S, Tang J, et al. Variation in community structure of arbuscular mycorrhizal fungi associated with a Cu tolerant plant—Elsholtzia splendens[J]. Applied Soil Ecology, 2010, 44(3): 191-197.
[28]Hassan S E, Boon E E, Starnaud M, et al. Molecular biodiversity of arbuscular mycorrhizal fungi in trace metal-polluted soils[J]. Molecular Ecology, 2011, 20(16): 3469-3483.