Abstract: This study introduced the breeding process of the new high protein soybean variety‘Qindou 2014’. We used the self-cultivating high-protein line 96E218 as the female parent and the high-yield variety‘Pudou 10’as the male parent for sexual cross-breeding. The offsprings were separated and selected by pod-picking mixing method. Through the product comparison test, regional test and production test, it proved that‘Qindou 2014’had the following characteristics, high yield and stable yield, strong disease resistance, wide adaptability and high protein content (47.79% crude protein content). After sowing date and density test, the best sowing date and the optimum density of the variety were determined. Finally, we also discussed the breeding methods and promotion models of high-protein soybean varieties.