Abstract: With 2-year-old clones hardwood of Sambucus williamsii Hance as test materials, the optimal rooting agent and concentrations are discussed about hard branch cuttings in Sambucus williamsii Hance.Test results show that:The average rooting rates of Sambucus williamsii hardwood cuttings was 62.5%, 71.6% and 73.4%, respectively,under the treatment of rooting agents ABT1, NAA, IBA, among them,?200μg/mL of NAA was treated for 2h, and the average cutting rooting rate was 80.6%; ?The rooting effect of the lower part of the branch (80.5%) and the middle part (78.3%) was significantly better than that of the upper part (51.4%);no matter what kind of rooting agent,the average root length and number of roots of the upper cuttings were smaller than the middle and lower parts;?The average number of leaves in different parts of the cuttings did not change much,while a larger change in length of the branch?, the lower cuttings were treated with 200μg/mL NAA for 2h, and the average length was 15.75 cm.?Hardwood cutting of Sambucus williamsii Hance dominated by callus rooting, callus rooting type belongs to.?Based on the combination of rooting rate and growth character, the efficiency of hardwood cutting propagation was evaluated comprehensively in Sambucus williamsii Hance，?The optimum rooting agent type and concentration was 100μg/mL for NAA treatment for 1h.The conclusion of the study is of great significance to improve the propagation coefficient of hardwood cuttings of Sambucus williamsii Hance.