Abstract: The study aims to understand theVibrio distribution characteristics ofLitopenaeus vannamei and the molecular genetic characteristics of main species in Shanghai, and provide a basis for the investigation and prevention of vibriosis in the future. The isolation and identification ofVibrio fromLitopenaeus vannamei was studied by selective medium separation and automatic identification system of bacteria. 52 strains were isolated from different fishponds and different regions. Among these strains, 30 strains were Vibrio parahaemolyticus (57.7%), 9 strains wereVibrio mimicus (17.3%), 7 strains wereVibrio vulnificus (13.5%), 4 strains wereVibrio alginolyticus (7.7%), 1 strain wasVibrio cholerae (1.9%) and 1 strain wasVibrio fluvialis (1.9%). The results of the investigation showed thatVibrio parahaemolyticus was the main type ofvibrios which was isolated fromLitopenaeus vannamei in Shanghai. The genetic typing of 30Vibrio parahaemolyticus strains carried out through ERIC, ERIC-PCR finger print revealed that the genotypes had abundant genetic diversity. And every strain was amplified 4-8 bands within 250-4500 bp. 5 cluster groups were divided by cluster analysis, including 15 strains of Ⅰcluster group（50%）, 6 strains of Ⅱ cluster group (20%), 2 strains of Ⅲ cluster group (6.7%), 2 strains of Ⅳ cluster group (6.7%), and 1 strain ofⅤ cluster group (3.3%). The results indicated thatV. parahamolyticus of Ⅰcluster group was popular in Shanghai.