Fine Mapping of Anther Color Gene Siac1 in Foxtail Millet

doi:10.11924/j.issn.1000-6850.casb18110025

Abstract

Abstract: To reveal the genetic basis of anther color in foxtail millet at molecular level, an F2 segregating population derived from a crossing between‘E1005’(female parent) and‘Pinzi 39’(male parent) was used as mapping population. With bulked segregation analysis (BSA) and recessive-class analysis (RCA), the Siac1 related to anther color was finely mapped. Genetic analysis showed that the segregation ratio of yellow anther plants to white anther plants was consistent with the Mendelian segregation of 3:1 in the F2 generation, which suggested that the white anther color trait was controlled by a pair of recessive nuclear genes. The Siac1 was preliminary mapped onto an interval of about 282 kb between markers GSA07025 and GSA07037 on chromosome VI by SSR markers and SV markers. Furthermore, fine mapping of Siac1 was conducted using newly developed InDel markers based on the sequence differences between the two parents, and the results indicated that the Siac1 was narrowed down onto a genomic region of around 94.7 kb between markers MRI579 and MRI583. Bioinformatics analysis showed that there were 10 Open Reading Frames (ORFs), and Seita.6G227100, eita.6G227200, Seita.6G227300 and Seita.6G227900 could be speculated to be four candidates for Siac1. This research lays a foundation for the cloning of Siac1 and the analysis of developmental mechanism of anther color.

CLC Number:

S515

郭二虎. Fine Mapping of Anther Color Gene Siac1 in Foxtail Millet[J]. Chinese Agricultural Science Bulletin, 2019, 35(12): 130-136.

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