The article summarized a few factions of Ribonuclease in the Plant, such as resistance, senility, and Self-incompatibility, male-sterile and so on. As a specific promoter such as TA29, OsgB6 and a RNase gene-barnase from Bacilus amyloliquefciens for the construction of msle-sterile chimaeric gene that be introduced to plant, we will get transformed plant which are complete male sterile .So researchers value the study of barnase.