The flower of Tetraploidy Robinia pseudoacacia for fodder can be anatomized into many Components that consist of specialized cells that may lead the Components to be different from each other in tissue culture. By inoculating the Components on the culture medium(MS) with different Concentration of hormone the following primary trends are found out: the whole Components can be divided into two groups according to their traits in culture; group 1, including all Components from outside calyx and calyx, differentiate relatively easily but have a high contamination rate; group 2, involving all Components from inside calyx, differentiate relatively difficultly but own a low contamination rate.