The effect of the prebiotic chicken’s performance and lymphoid organ’s developing were studied in the experiment.100 one- day age chickens were selected in this experiment and was divide into random contrast group and experiment group, 50/group.the basic diet is the same, prebiotics of 500g/t added to the basic diet. at end the experiment ,10 chick’s were setected every groups and kill them to take out of thymu ,bursas of fabricius and spleen ,the samples were fixded in the Bouin liquid , produce the slice and H?E dyeing .the slice were observe and photograph under the micnoscope of OLYMPUS. The result reveals that the experiment group increases were weight gain day than the contrast group. The difference is remarkinber (p <0.05), while day food yield is not remarkinber (p >0.05), the rate of food to meat in experimented group is less them the contract group, In the experiment group ,the cortex of thymic lobule ,bursa fibrous and Periarterial Lymphatic Sheaths thicken obviously splenic nodule and ellipsoid augmented, the plica of bursas of Fabricious developed well and the degenerating of it retarded . The result shows that the Prebiotics could enhance the early development of thymus and bursas of Fabricius,retard the degenerating of bursas of Fabricius ,and promote the immune response of spleen .