Abstract: A series of factors affecting rooting were studied in embryo culture of Chinese Date(Popozao).The results of experiment are as follows: IAA was more effective than IBA and NAA in stimulating adventitious rooting regeneration, reached as high as 60.7% of rooting rate when IAA is 2.0mg/L;But,the coordinating result of IAA and IBA is better than that of IAA and IBA by single, the best compose is IBA 1.0mg/L and IAA 0.01mg/L,and the best medium of inducting rooting is 1/2MS+IBA1.0mg/L+IAA0.01mg/L+sugar20g,91.0% rooting rate obtained;Cytokinin BA restrain the formation of adventitious rooting; Other factors of affecting rooting, such as the quality of vitro seedling and dark time, were described, too. Key word: Chinese Date; embryogenic test tube seedling; rooting