Abstract: Using 4 clones donor of triploid Chinese white poplar as materials, the effects of different hormone combinations, content of macroelements and additives (coconut water, banana and potato) in medium on propagation coeifficient and bud-vitrifiation were compared. The results showed that the frequency of shoot-induction was increased by supplemented with 6-BA 0.5mg/L and NAA 0.01mg/L in MS medium and the frequency of bud-vitrifiation was decreased by modifying MS medium(2/3 of macroelements). The higher propagation coeifficient and lower frequency of bud-vitrifiation could be obtained by using 2/3MS + 0.5mg/L 6-BA + 0.01mg/L NAA + 150g/L coconut water. The difference of propagation coeifficient and frequency of bud-vitrifiation among tested clones were not obvious.