Abstract: The zymotic production by ferment cultivate and extraction from Tri-BA81 of Verticillium lecanii introduced from institute of Plant Protection of Russia, is applied to prevent five aphis. The result shows, the zymotic production has high poison effects. Biometrics indoor, 1% liquids of it have 76.35% and 91.53% death rate on Breviloryne bvassicae and Aphis gossypii, 2% liquids of it have 91.79% death rate on Myzus persicae. 1% and 3% liquids of it have 65.45% and 100% death rate on Macrosiphum avenae, and have 65.56% and 99.56% death rate on Aphis glycines; have 72.5% and 79.4% death rate in field.