In the present paper, 20 strains of Paecilomyces lilacinus from different geographic origins and other 4 species of Paecilomyces were studied by random amplified polymorphic DNA(RAPD). Distinct genetic diversity among 21 strains of Paecilomyces lilacinus were found. DNA fingerprinting with RAPD-PCR provides high resolution of genotype distribution. Genetic variability of different strains of paecilomyces lilacinus is related to genetic type, and not to geographic origins. Genetic variability among different strains of paecilomyces lilacinus were more than different species, maybe there was clostered species.