Five media were adopted to study the effects of three plant growth regulators (GA3、NAA、BAP) on the micropropagation of five Virus-free tube potato plantlets. The results showed:The number ③ medium (MS+1mg/L GA3+0.1mg/L NAA) is suitable to the micropropagation of Kexin No.4 、Kexin No.13 and Youjin; The number ④ medium(MS+0.01mg/L BAP+0.05mg/L NAA) is suitable to the micropropagation of Zaodabai; However, The number ① medium (MS medium ), which has no plant growth regulators, is suitable to the micropropagation of Kexin No.16. From this study we hope to lay solid foundation of low cost rapid and better micropropagation of Virus-free tube potato plantlets.