Abstract: To improve the keep fresh quality of Agaricus blazei, different manipulation was used to keep fresh and keep fresh for this study. An orthogonal experiment was carried out to investigate the effects. Browning and diet cellulose of Agaricus blazei in keep fresh was analyzed. The result showed that when deal properly with Agaricus blazei, the browning was alleviated and the quality changes has slowed at the keep fresh period. Under the same conditions, steep Agaricus blazei in the manipulation of 0.25% NaHSO3 30 min, dredge up Agaricus blazei from the aqueous solution and water content of Agaricus blazei was lost, mix up the aqueous solution of 0.15%VC with 0.5% citric acid to steep 10min again, the effect of keep fresh was better. A regression analysis of Agaricus blazei by those as keep fresh was used and a mathematical model was obtained.