Abstract: Coptis chinensis Franch of 1,2,3,4 and 5-age years were given treatment of high temperature (35±2)℃for 0d、3ds and 6ds. the contents of soluble sugar, free proline and MDA in leaves and roots of Coptis chinensis Franch of different years of growth is compared。The results showed that high temperature stress increased the content of soluble sugar and free proline, the soluble sugar had accumulated 1 times more than control and proline 7 times uner high temperature for 6ds. however, the content of MDA decreased in both leaves and roots. We researched the effects of high temperature stress on physiological and biochemical characteristics of Coptis chinensis Franch ,which provides some theory guidance for production.