Abstract: Studies on types of freeze injury and diagnostic characteristics in the wheat production region located in the reaches of Yellow River and the Huaihe River and the middle and lower Yangtze River. We put forward some prevention measures of defense freeze injury of winter wheat, such as breeding and adopting cold resistance varieties, improving reasonable distribution of wheat varieties, rational planning sowing time and seed quantity according to wheat varieties vernalization characteristics, improving soil tillage and seed sowing quality, improving the quality of seedling before live through the winter, irrigating water in order to preventing frost freeze injury, Scientific top application of rapid Efficiency nitrogen, et al. On the bases, applying corresponding salvage measures according to difference freeze injury seedlings condition.