The survival rate of Chinese Cherry‘Duiyingtao’（Prunus pseudocerasus）tube seedling that transplant to nutritional soil directly was 0%. The successful transplantation was gained by the method that acclimate tube seedling in free soil medium in a little plastic hut in greenhouse at first transplantation, then transplant in nutritional soil. The survival rate raise to over 95%.The results show that relative air humidity, temperature in acclimatization and drainage of nutritional soil in transplantation are main factors to ensure successful transplantation. If the relative air humidity was kept 90%~100%, then more high survival rates would be attained. The fitting transplant air temperature was between 22~26℃ and the fitting medium was 15-18℃, and the fitting medium was vermiculite or vermiculite and perlite mixture in volume of 1:1. After 40 days of Accommodation, the seedlings were transplanted to nutritional soil that was made from mixture of loam, sand and peat in dispensation of 4:2:1 in volume. It can efficiently drain excess water out of pot after transplantation by putting the pots on the earth. The survival rate of transplantation was 95% when 50% excess water was drained out of pots in 3~5 days.
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