Effects of 1-MCP Combined with MAP on Softening and Cell Wall Metabolism of ‘Kongxin’ Plum

doi:10.11924/j.issn.1000-6850.casb20190700388

Abstract

Abstract:

The objective is to explore the relationship between the fruit softening and cell wall metabolism and fruit softening regulatory mechanism of Prunus salicina ‘Kongxin’ by 1-methylcycropene (1-MCP), modified atmosphere packaging (MAP) and the combination of 1-MCP and MAP, and to provide technical references for solving the problem of plum fruit softening after harvesting. With P. salicina ‘Kongxin’ fruits as experimental materials, the changes of firmness, cell wall compositions, the metabolism-related enzyme activities under 1-MCP, MAP, and 1-MCP+MAP treatment during storage at (20±1)℃ for twelve days were investigated. The results were as follows: (1) compared with control (placed directly in carton), 1-MCP, MAP and 1-MCP+MAP delayed the decrease of fruit firmness and the degradation of protopectin and cellulose, and inhibited the enzyme activities of polygalacturonase (PG), pectin methylesterase (PME) and cellulase (Cx), the inhibitory effect of the treatment was 1-MCP+MAP>1-MCP>MAP; (2) the decrease of fruit firmness was obviously and positively correlated with the content of protopectin and cellulose, and was not consistent with the change of soluble pectin content; 1-MCP and 1-MCP+MAP treatment were negatively correlated with soluble pectin content, while the control and MAP treatment showed no significant correlation with soluble pectin content; there was a significantly negative correlation between the decrease of fruit firmness under 1-MCP, MAP, 1-MCP+MAP and control treatment with PG and PME activities, but there was a mixed relationship between the fruit firmness and Cx activity, including a significantly negative correlation in the control and MAP treatment, while no significant correlation in 1-MCP and 1-MCP+MAP treatment. The results indicated that 1-MCP combined with MAP treatment could significantly inhibit the firmness decline, the degradation of cell wall substances and the enzyme activities in the process of softening, to control the softening of P. salicina ‘Kongxin’ fruits, prolong the storage and reduce the loss after postharvest.

Key words: Prunus salicina ‘Kongxin’, 1-methylcyclopropene, modified atmosphere packaging, fruit softening, cell wall metabolism

CLC Number:

S662.3

Dong Xiaoqing, Liu Hongtan, Zhu Shouliang, Wang Xiaohong, Chen Hong, Hu Xiaojing. Effects of 1-MCP Combined with MAP on Softening and Cell Wall Metabolism of ‘Kongxin’ Plum[J]. Chinese Agricultural Science Bulletin, 2020, 36(22): 129-135.

Figures/Tables 8

References 27

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处理	测定指标	硬度	原果胶	可溶性果胶	纤维素
CK	多聚半乳糖醛酸酶(PG)	-0.982^**	-0.957^**	0.193	-0.962^**
	果胶甲脂酶活性(PME)	-0.895^**	-0.938^**	-0.083	-0.904^**
	纤维素酶活性(Cx)	-0.842^*	-0.882^**	-0.249	-0.838^*
	硬度	-	0.973^**	-0.288	0.975^**
1-MCP	多聚半乳糖醛酸酶(PG)	-0.827^*	-0.890^**	0.441	0.899^**
	果胶甲脂酶活性(PME)	-0.763^*	-0.973^**	0.263	-0.769^*
	纤维素酶活性(Cx)	-0.639	-0.913^**	0.104	-0.713^*
	硬度	-	0.815^*	-0.765^*	0.976^*
MAP	多聚半乳糖醛酸酶(PG)	-0.832^*	-0.881^*	-0.020	-0.835^*
	果胶甲脂酶活性(PME)	-0.821^*	-0.876^**	-0.130	-0.797^*
	纤维素酶活性(Cx)	-0.861^*	-0.877^**	-0.301	-0.794^*
	硬度	-	0.961^**	-0.220	0.958^**
1-MCP+MAP	多聚半乳糖醛酸酶(PG)	-0.757^*	-0.798^*	0.296	-0.883^**
	果胶甲脂酶活性(PME)	-0.816^*	-0.882^**	0.528	-0.711
	纤维素酶活性(Cx)	-0.657	-0.694	0.367	-0.756^*
	硬度	-	0.980^**	-0.878^**	0.972^**

处理	测定指标	硬度	原果胶	可溶性果胶	纤维素
CK	多聚半乳糖醛酸酶(PG)	-0.982^**	-0.957^**	0.193	-0.962^**
	果胶甲脂酶活性(PME)	-0.895^**	-0.938^**	-0.083	-0.904^**
	纤维素酶活性(Cx)	-0.842^*	-0.882^**	-0.249	-0.838^*
	硬度	-	0.973^**	-0.288	0.975^**
1-MCP	多聚半乳糖醛酸酶(PG)	-0.827^*	-0.890^**	0.441	0.899^**
	果胶甲脂酶活性(PME)	-0.763^*	-0.973^**	0.263	-0.769^*
	纤维素酶活性(Cx)	-0.639	-0.913^**	0.104	-0.713^*
	硬度	-	0.815^*	-0.765^*	0.976^*
MAP	多聚半乳糖醛酸酶(PG)	-0.832^*	-0.881^*	-0.020	-0.835^*
	果胶甲脂酶活性(PME)	-0.821^*	-0.876^**	-0.130	-0.797^*
	纤维素酶活性(Cx)	-0.861^*	-0.877^**	-0.301	-0.794^*
	硬度	-	0.961^**	-0.220	0.958^**
1-MCP+MAP	多聚半乳糖醛酸酶(PG)	-0.757^*	-0.798^*	0.296	-0.883^**
	果胶甲脂酶活性(PME)	-0.816^*	-0.882^**	0.528	-0.711
	纤维素酶活性(Cx)	-0.657	-0.694	0.367	-0.756^*
	硬度	-	0.980^**	-0.878^**	0.972^**