(In order to getting)To search an antibacterial potato (Solanum tuberosum L.), Various conditions for potato transformation with Agrobacterium tumefaciens LBA4404 were investigated and optimal conditions for the cultivar “ Taiwanhong” were founded. At the same time, a efficient transformation system was developed. 1)The D600 of the Agrobacterium suspension was adjusted to 0.5~0.8 and then stems and leaf discs were dropped into the suspension for 5 min. 2)Stems and leaves were pre-cultured for 4 days and 8 days respectively. 3)The duration of co-culture is 3 days. Though this system SPCEMA gene was introduced into “Taiwanhong” and this was confirmed by PCR and Southern blotting. The transformation frequency of the stems and leaves was 10.00% and 5.79% respectively.
